CD27 Antibody / HuProt Proteome-Wide Specificity Validated Antibody [clone LPFS2/4179] (V8726)

CD27 Antibody Specificity Validated IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human tonsil tissue using HuProt Proteome-Wide Specificity Validated CD27 Antibody clone LPFS2/4179. Strong membranous HRP-DAB brown staining is observed in lymphocyte populations within tonsillar germinal centers and interfollicular regions, consistent with CD27 expression on activated and memory T and B cells, while surrounding non-lymphoid elements remain largely negative. The staining pattern supports selective target recognition across complex tissue environments and highlights immune cell compartmentalization within lymphoid tissue. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human tonsil with CD27 antibody (clone LPFS2/4179). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD27 Antibody with HuProt Validation. HuProt proteome-wide specificity analysis of CD27 / TNFRSF7 using HuProt Proteome-Wide Specificity Validated CD27 Antibody clone LPFS2/4179 on an array containing more than 19,000 full-length human proteins. CD27 shows the highest signal intensity with a Z-score of 155.71 and S-score of 148.14, indicating highly selective binding relative to all other proteins on the array. The Z-score reflects signal strength above the array mean, while the S-score represents the separation between the top-ranked target and the next highest signal, supporting strong target specificity and minimal cross-reactivity across the human proteome.