CD27 Antibody / Antigen-Experienced Lymphocyte Marker Antibody [clone LPFS2/4178] (V9436)

CD27 Antibody for IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human tonsil tissue using CD27 Antibody clone LPFS2/4178. Membranous HRP-DAB brown staining is observed in scattered lymphocyte populations within interfollicular and paracortical regions, consistent with CD27 expression on antigen-experienced and activated T and B cells, while adjacent areas show lower staining intensity. This staining pattern is characteristic of an antigen-experienced lymphocyte marker and supports evaluation of immune activation history within tonsillar tissue architecture. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human tonsil tissue with CD27 antibody (clone LPFS2/4178). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD27 antibody (clone LPFS2/4178). These results demonstrate the foremost specificity of the LPFS2/4178 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

CD27 Antibody for WB. Western blot analysis of CD27 / TNFRSF7 expression in human tonsil tissue using CD27 Antibody. Lane 1: human tonsil lysate. A band is detected at approximately 50–60 kDa, consistent with glycosylated CD27, noting a lower predicted molecular weight of approximately 29 kDa for the core protein. CD27 is a glycosylated receptor, and the higher apparent molecular weight reflects N-linked glycosylation commonly observed in lymphocyte-rich tissues. The detection pattern is consistent with expression in antigen-experienced lymphocyte populations and supports its use as an antigen-experienced lymphocyte marker in western blot analysis.

SDS-PAGE analysis of purified, BSA-free CD27 antibody (clone LPFS2/4178) as confirmation of integrity and purity.