CD27 Antibody / Protein Microarray Specificity Validated Antibody [clone LPFS2/4177] (V8725)

CD27 Antibody for IHC. Immunohistochemistry analysis of CD27 / TNFRSF7 expression in FFPE human tonsil tissue using Protein Microarray Specificity Validated CD27 Antibody clone LPFS2/4177. Strong membranous HRP-DAB brown staining is observed in lymphocyte populations within tonsillar germinal centers and interfollicular regions, consistent with CD27 expression on activated and memory T and B cells, while surrounding non-lymphoid structures remain largely negative. The staining pattern supports selective target recognition and highlights immune cell compartmentalization within lymphoid tissue. Heat-induced epitope retrieval was performed in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human tonsil with CD27 antibody (clone LPFS2/4177). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with CD27 antibody (clone LPFS2/4177). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

CD27 Antibody Microarray Validation. Protein microarray specificity analysis of CD27 / TNFRSF7 using Protein Microarray Specificity Validated CD27 Antibody clone LPFS2/4177 on a HuProt array containing more than 19,000 full-length human proteins. CD27 shows the highest signal intensity with a Z-score of 155.61 and S-score of 145.21, indicating highly selective binding relative to all other proteins on the array. The Z-score reflects signal strength above the array mean, while the S-score represents the separation between the top-ranked target and the next highest signal, supporting strong target specificity and minimal cross-reactivity.

SDS-PAGE analysis of purified, BSA-free CD27 antibody (clone LPFS2/4177) as confirmation of integrity and purity.

Western blot testing of human Raji and Ramos cell lysate with CD27 antibody. Predicted molecular weight ~29 kDa but may be observed at higher molecular weights due to glycosylation.