- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD27 Antibody / High Specificity CD27 Antibody [clone LPFS2/4176] (V9435)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V9435-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V9435-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9435SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | LPFS2/4176 |
| Purity | Protein A/G affinity |
| UniProt | P26842 |
| Localization | Cell Surface |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml Western Blot : 2-4ug/ml |
| Limitations | This CD27 Antibody / High Specificity CD27 Antibody is available for research use only. |
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CD27, also known as TNF receptor superfamily member 7 (TNFRSF7), is a type I transmembrane receptor expressed on T lymphocytes, memory B cells, and subsets of natural killer cells, where it regulates immune activation, survival, and differentiation. CD27 Antibody / High Specificity CD27 Antibody (clone LPFS2/4176) is uniquely positioned for applications requiring strong target selectivity with controlled cross-reactivity, supported by protein array-based validation demonstrating clear enrichment of CD27 signal relative to other proteins. CD27 antibody reagents are widely used in immunology and cell signaling studies, where specificity directly impacts the accuracy of downstream data interpretation.
CD27 antibody, also referred to as TNFRSF7 antibody or CD27 immune receptor antibody in the literature, belongs to the TNF receptor superfamily, a group of structurally related proteins that share conserved extracellular domains. This structural similarity presents a known challenge for antibody specificity, as closely related receptors can produce overlapping or non-specific signals if not carefully distinguished. Antibodies with demonstrated target selectivity are therefore critical for confidently resolving CD27 expression in complex biological systems.
CD27 Antibody / High Specificity CD27 Antibody (clone LPFS2/4176) demonstrates a dominant signal for CD27 in protein array analysis, with a marked drop in signal intensity across lower-ranked proteins. This distribution supports selective target recognition and indicates minimal off-target binding under the tested conditions. The clear separation between CD27 and secondary signals provides practical assurance of specificity while maintaining robust detection sensitivity, making this antibody well suited for applications where both signal strength and selectivity are required.
In contrast to broad panel or proteome-wide validation approaches, this level of specificity assessment focuses on signal hierarchy and relative enrichment, offering a balanced evaluation of antibody performance. The observed signal profile supports reliable discrimination of CD27 from related proteins within the TNF receptor family, reducing the likelihood of cross-reactive detection in downstream applications.
In biological samples, CD27 is expressed in immune cell populations with dynamic activation states and undergoes post-translational modifications, including glycosylation. These factors can increase sample complexity and elevate the importance of antibody selectivity. High-specificity antibodies help ensure that detected signals correspond to CD27 rather than co-expressed or structurally related proteins, improving confidence in experiments involving immune profiling, signaling analysis, and protein expression studies.
The mouse monoclonal clone LPFS2/XXXX antibody is designed to provide consistent and reproducible detection of CD27, with validation data supporting strong target enrichment and low background signal. This makes it particularly useful for studies involving complex lysates, comparative analyses, or experimental systems where reducing non-specific binding is essential for accurate interpretation.
Overall, CD27 Antibody / High Specificity CD27 Antibody (clone LPFS2/4176) provides reliable detection of CD27 with a well-defined specificity profile, supported by protein array signal separation that demonstrates strong target enrichment and reduced off-target interaction in research applications.
This antibody is part of a broader CD27 antibody collection designed to support diverse immunological research applications.
Optimal dilution of the CD27 Antibody / High Specificity CD27 Antibody should be determined by the researcher.
A portion of amino acids 28-170 was used as the immunogen for the CD27 antibody.
Aliquot the CD27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
TNFRSF7 antibody, CD27 high specificity antibody, CD27 low cross reactivity antibody, CD27 validated antibody, CD27 monoclonal antibody
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