CD25 Antibody IL2RA/2393 / IL2RA Monoclonal Antibody [clone IL2RA/2393] (V8512)

CD25 Antibody IL2RA/2393 Detects IL2RA in Ramos, HeLa and Jurkat Cells WB. Western blot analysis of human Ramos B lymphocyte, HeLa cervical cancer, and Jurkat T lymphocyte cell lysates using CD25 Antibody clone IL2RA/2393 detects a strong, discrete band at approximately 36 kDa in all three samples, with minimal additional background. CD25 is a glycosylated cell-surface protein whose electrophoretic migration can vary with glycosylation state. The consistent band across three distinct human cell lines demonstrates detection of IL2RA by this IL2RA Monoclonal Antibody under Western blot conditions.

SDS-PAGE analysis of purified, BSA-free CD25 antibody (clone IL2RA/2393) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD25 antibody (clone IL2RA/2393). These results demonstrate the foremost specificity of the IL2RA/2393 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.