CD243 Antibody / Multidrug Resistance 1 / ABCB1 [clone ABCB1/9309] (V5969)

Western blot analysis of CD243 / Multidrug Resistance 1 antibody in human tissues. Western blot was performed using CD243 / Multidrug Resistance 1 antibody (clone ABCB1/9309) on human kidney and human liver tissue lysates. A strong immunoreactive band is detected at approximately 170 kDa in both samples, consistent with the predicted molecular weight of ABCB1, also known as p170 or P-glycoprotein. The band appears broad and slightly diffuse, which is consistent with the extensively glycosylated nature of Multidrug Resistance 1 and its membrane-associated processing. The detected signal corresponds to the predicted molecular weight under reducing conditions. ABCB1 is physiologically enriched in kidney proximal tubules and liver canalicular membranes, and the prominent band intensity in these tissues supports specific detection of endogenous CD243.

Western blot analysis of CD243 / Multidrug Resistance 1 antibody in human kidney. Western blot was performed using CD243 / Multidrug Resistance 1 antibody (clone ABCB1/9309) on human kidney tissue lysate. A prominent immunoreactive band is detected at approximately 170 kDa, consistent with the predicted molecular weight of ABCB1, also known as p170 or P-glycoprotein. The band displays a slightly diffuse appearance, which is consistent with the heavily glycosylated nature of Multidrug Resistance 1 and its membrane-associated processing. The detected signal corresponds to the predicted molecular weight under reducing conditions. Strong expression in kidney tissue aligns with the known enrichment of ABCB1 in proximal tubular epithelial cells, supporting specific detection of endogenous CD243.

Immunohistochemistry analysis of CD243 / Multidrug Resistance 1 antibody in human adrenal gland. FFPE human adrenal gland tissue was stained with CD243 / Multidrug Resistance 1 antibody (clone ABCB1/9309). HRP-DAB brown chromogenic signal is observed predominantly along the plasma membrane of adrenal cortical cells, producing a distinct membranous staining pattern consistent with the known localization of the p170 ABCB1 drug efflux transporter. Cortical cell populations demonstrate variable membrane-associated staining intensity, while stromal elements show minimal background signal. The inset negative control using PBS instead of primary antibody shows no specific brown staining. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature prior to antibody incubation.

SDS-PAGE Analysis of Purified CD243/Multidrug Resistance 1 antibody (ABCB1/9309). Confirmation of Purity and Integrity of Antibody.