CD23 Antibody - Microarray Validation [clone FCER2/6890] (V8871)

IHC staining of FFPE human colon tissue with CD23 antibody (clone FCER2/6890) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with CD23 antibody (clone FCER2/6890) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD23 antibody (clone FCER2/6890) as confirmation of integrity and purity.

Protein microarray validation of CD23 Antibody - Microarray Validation (clone FCER2/6890). Analysis of a protein array containing more than 19,000 full-length human proteins was performed using CD23 antibody clone FCER2/6890. The antibody demonstrates highest reactivity with FCER2, confirming specific recognition of CD23, with substantially lower signal intensity observed for non-target proteins. These results support the target specificity of the FCER2/6890 monoclonal antibody.
Z- and S-score explanation: The Z-score represents the strength of the fluorescent signal generated when the monoclonal antibody binds to a specific protein on the HuProt array, expressed as standard deviations above the mean signal of all proteins on the array. When proteins are arranged in descending order according to Z-score, the S-score is calculated as the difference in Z-scores between adjacent ranked proteins. The S-score therefore reflects the relative specificity of the monoclonal antibody for its intended target compared to other proteins on the array. An antibody is generally considered specific for its intended target when the S-score is at least 2.5.