CD23 Antibody - HuProt Validated [clone FCER2/6887] (V9384)

Immunohistochemistry of CD23 Antibody in human tonsil. FFPE human tonsil tissue was stained with CD23 antibody (clone FCER2/6887) at 2 ug/ml in PBS for 30 minutes at room temperature. Prominent membranous HRP-DAB brown staining is observed in B lymphocytes within germinal centers and mantle zone regions, consistent with known CD23 expression on mature follicular B cells. The staining pattern highlights follicular architecture, with dense membrane labeling in B cell rich areas and minimal staining in adjacent T cell predominant interfollicular zones. The negative control inset shows tissue processed with PBS in place of primary antibody, demonstrating absence of non-specific secondary antibody binding. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation. Clone FCER2/6887 is supported by human protein microarray validation demonstrating target specificity.

Immunohistochemistry of CD23 Antibody in human lymph node. FFPE human lymph node tissue was stained with CD23 antibody (clone FCER2/6887) at 2 ug/ml in PBS for 30 minutes at room temperature. Distinct membranous HRP-DAB brown staining is observed in B lymphocytes within follicular germinal centers, consistent with established CD23 expression on mature follicular B cells. The staining pattern highlights nodal follicular architecture, with strong membrane labeling in B cell rich regions and minimal staining in surrounding paracortical T cell areas. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation. Clone FCER2/6887 is supported by human tissue protein microarray validation demonstrating target specificity.

HuProt microarray analysis was performed to evaluate the specificity of CD23 antibody (clone FCER2/6887). The array contains more than 19,000 full-length human proteins. The antibody demonstrates highest reactivity with FCER2, confirming selective recognition of CD23, with substantially lower signal intensity observed for non-target proteins. These results support the specificity of the FCER2/6887 monoclonal antibody.
Z- and S-score explanation: The Z-score represents the strength of the fluorescent signal generated when the antibody binds to a specific protein on the HuProt array, expressed in units of standard deviations above the mean signal of all proteins on the array. When proteins are arranged in descending order according to Z-score, the S-score is calculated as the difference in Z-scores between sequentially ranked proteins. The S-score therefore reflects the relative specificity of the antibody for its intended target compared to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free CD23 antibody (FCER2/6887) as confirmation of integrity and purity.