CD23 Antibody Biotin Conjugate [clone FCER2/3592] (V7565BTN)

Immunohistochemistry of CD23 Antibody Biotin Conjugate in human tonsil. FFPE human tonsil tissue was stained with biotin-labeled CD23 antibody (clone FCER2/3592). Prominent membranous HRP-DAB brown staining is observed in B lymphocytes within germinal centers and mantle zone regions, consistent with established CD23 expression on mature follicular B cells. The staining pattern highlights follicular architecture, with dense labeling in B cell rich areas and minimal staining in adjacent T cell predominant interfollicular zones. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 20 minutes followed by cooling prior to antibody incubation. The biotin conjugate format allows detection using streptavidin based amplification systems.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD23 antibody (clone FCER2/3592). These results demonstrate the foremost specificity of the FCER2/3592 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD23 antibody (clone FCER2/3592) as confirmation of integrity and purity.