CD23 Antibody - Protein Microarray Validated [clone FCER2/3592] (V7565)

Immunohistochemistry of CD23 Antibody in human tonsil. FFPE human tonsil tissue was stained with CD23 antibody (clone FCER2/3592). Distinct membranous HRP-DAB brown staining is observed in B lymphocytes within germinal centers and mantle zone regions, consistent with known CD23 expression on mature follicular B cells. The staining pattern highlights follicular architecture, with dense positivity in B cell rich areas and minimal staining in surrounding T cell predominant interfollicular regions. Heat induced epitope retrieval was performed by boiling tissue sections in pH 6 citrate buffer for 10-20 minutes followed by cooling prior to antibody incubation.

Protein microarray validation of CD23 Antibody - Protein Microarray Validated (clone FCER2/3592). Analysis of the HuProt microarray containing more than 19,000 full-length human proteins was performed using CD23 antibody clone FCER2/3592. The antibody demonstrates highest reactivity with FCER2, confirming target specificity, with markedly lower signal intensity observed for non-target proteins. These results support the specificity of the FCER2/3592 monoclonal antibody for CD23.
Z- and S-score explanation: The Z-score represents the strength of the fluorescent signal generated when the antibody binds to a specific protein on the HuProt array, expressed as standard deviations above the mean signal of all proteins on the array. When proteins are ranked in descending order by Z-score, the S-score represents the difference in Z-scores between sequentially ranked proteins. The S-score therefore reflects the relative specificity of the antibody for its intended target compared to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free CD23 antibody (clone FCER2/3592) as confirmation of integrity and purity.