CD23 Antibody / B-Cell Activation and IgE Receptor Marker [clone FCER2/3592] (V7565)

CD23 Antibody Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue stained with CD23 antibody clone FCER2/3592. Distinct membranous and cytoplasmic HRP-DAB brown staining is observed in germinal center-associated B lymphocytes and follicular dendritic cell networks, consistent with the established expression pattern of Low-affinity immunoglobulin epsilon Fc receptor / FCER2/CD23 in reactive lymphoid follicles. The staining pattern highlights follicular architecture with strong positivity in B-cell-rich germinal center regions and comparatively minimal staining in surrounding interfollicular T-cell areas. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH6 citrate buffer for 10-20 min followed by cooling before antibody incubation.

Protein microarray validation of CD23 Antibody / B-Cell Activation and IgE Receptor Marker (clone FCER2/3592). Analysis of the HuProt microarray containing more than 19,000 full-length human proteins was performed using CD23 antibody clone FCER2/3592. The antibody demonstrates highest reactivity with FCER2, confirming target specificity, with markedly lower signal intensity observed for non-target proteins. These results support the specificity of the FCER2/3592 monoclonal antibody for CD23.
Z- and S-score explanation: The Z-score represents the strength of the fluorescent signal generated when the antibody binds to a specific protein on the HuProt array, expressed as standard deviations above the mean signal of all proteins on the array. When proteins are ranked in descending order by Z-score, the S-score represents the difference in Z-scores between sequentially ranked proteins. The S-score therefore reflects the relative specificity of the antibody for its intended target compared to other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free CD23 antibody (clone FCER2/3592) as confirmation of integrity and purity.

CD23 Antibody Daudi WB. Western blot analysis of Daudi cell lysate using CD23 antibody clone FCER2/3592. A distinct band is detected at approximately 45 kDa, consistent with the predicted molecular weight of Low-affinity immunoglobulin epsilon Fc receptor / FCER2/CD23. The strong signal in this B-cell lineage cell line supports the established role of CD23 in mature B-cell function, IgE receptor signaling, and adaptive immune regulation associated with activated lymphoid populations.