CD226 Antibody / DNAM-1 Polyclonal Antibody (RQ6373)

CD226 Antibody by FACS in Rat NRK Cells. Flow cytometry analysis of rat NRK cells using CD226 Antibody at 1 ug per million cells. The DNAM-1 Polyclonal Antibody produces a pronounced rightward fluorescence shift (blue) compared with the isotype control (green) and unstained cells (red). The three populations are clearly separated, demonstrating detection of cell-associated CD226/DNAM-1 by flow cytometry. Cells were blocked with goat serum before antibody incubation to minimize nonspecific binding.

CD226 Antibody by IHC in Rat Lung Lymphoid Tissue. Immunohistochemistry of FFPE rat lung lymphoid tissue using CD226 Antibody. The DNAM-1 Polyclonal Antibody produces widespread brown DAB staining throughout the lymphoid tissue, with heterogeneous cellular labeling and comparatively limited nuclear staining. The staining is particularly prominent in densely populated cellular regions, consistent with the distribution of CD226-associated immunoreactivity within the tissue. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0.

CD226 Antibody by IHC in Mouse Lung. Immunohistochemistry of FFPE mouse lung sections using CD226 Antibody. Strong brown immunoreactivity is visible in cellular regions surrounding pulmonary airway and vascular structures, with additional staining in adjacent lung tissue. The DNAM-1 Polyclonal Antibody produces predominantly non-nuclear labeling, while the surrounding alveolar spaces remain unstained. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0 before antibody incubation.

CD226 Antibody by WB in Rodent Immune Tissues and Cells. Western blot testing of rat spleen (lane 1), mouse spleen (lane 2), mouse thymus (lane 3) and mouse RAW264.7 cell lysates (lane 4) using CD226 Antibody. The DNAM-1 Polyclonal Antibody detects prominent immunoreactive bands in the approximately 70-100 kDa region, with differences in band intensity and migration among the samples. The RAW264.7 lane shows multiple distinct bands in the upper portion of this range. The observed molecular weight variation is compatible with differences in CD226 glycosylation, although the presence of multiple bands does not independently establish their identities.