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Home >> Antibodies >> CD22 Antibody

CD22 Antibody [clone RFB4] (V8228)

  Catalog No Formulation Size Price (USD)  
Image V8228-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8228-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8228SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow cytometry testing of human Ramos cells with CD22 antibody (clone RFB4); Red=isotype control, Blue= CD22 antibody.
SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone RFB4) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name RFB4
Purity Protein G affinity chromatography
UniProt P20273
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Limitations This CD22 antibody is available for research use only.
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Description

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Application Notes

Optimal dilution of the CD22 antibody should be determined by the researcher.

Immunogen

Human tonsil lymphocytes were used as the immunogen for the CD22 antibody.

Storage

Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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