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Home >> Antibodies >> CD22 Antibody

CD22 Antibody [clone FR10B4] (V2984)

  Catalog No Formulation Size Price (USD)  
Image V2984-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2984-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2984SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name FR10B4
Purity Protein G affinity chromatography
UniProt P20273
Localization Cell surface, cytoplasmic
Applications Functional studies (order BSA/sodium azide-free format) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD22 antibody is available for research use only.
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Description

CD22 mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]

Application Notes

Optimal dilution of the CD22 antibody should be determined by the researcher.

Immunogen

Human Burkitt's lymphoma cells were used as the immunogen for the CD22 antibody.

Storage

Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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