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Home >> Antibodies >> CD22 Antibody / CD22 molecule

CD22 Antibody / CD22 molecule [clone MSVA-022R] (V6145)

  Catalog No Formulation Size Price (USD)  
Image V6145-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6145-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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B-cell receptor CD22 Rabbit Recombinant Monoclonal Antibody (MSVA-022R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-022R
UniProt P20273
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD22/CD22 molecule antibody is available for research use only.
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Application Notes

1. Optimal dilution of the CD22/CD22 molecule antibody should be determined by the researcher.

2. This CD22/CD22 molecule antibody is recombinantly produced by expression in CHO cells.

Immunogen

A recombinant fragment (around amino acids 52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary) was used as the immuongen for the CD22/CD22 molecule antibody.

Storage

CD22/CD22 molecule antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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