CD22 Antibody for IF / CD22 Immunofluorescence Antibody [clone BLCAM/1796] (V3784)

CD22 Antibody for IF. Immunofluorescence analysis of CD22/Siglec-2 expression in human Ramos B cell lymphoma cells using CD22 Immunofluorescence Antibody clone BLCAM/1796. CD22 staining (green) highlights prominent membrane-associated localization outlining B cell surfaces, consistent with its role as a B cell receptor regulatory protein, while Reddot nuclear stain (red) labels nuclei. The staining pattern supports identification of CD22-positive B cell populations and visualization of cell surface receptor distribution in lymphoma-derived cells.

Flow cytometry testing of human Ramos cells with CD22 antibody (clone BLCAM/1796); Red=isotype control, Blue= CD22 antibody.

Western blot testing of human Raji cell lysate with CD22 antibody (clone BLCAM/1796). Expected molecular weight: 76-150 kDa depending on glycosylation level.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD22 antibody (clone BLCAM/1796). These results demonstrate the foremost specificity of the BLCAM/1796 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD22 Antibody for IF / CD22 Immunofluorescence Antibody (clone BLCAM/1796) as confirmation of integrity and purity.