CD22 Antibody / CD22 Microarray Specificity Validated Antibody [clone BLCAM/1795] (V3693)

CD22 Antibody Tonsil IHC. Immunohistochemistry analysis of CD22/Siglec-2 expression in FFPE human tonsil tissue using CD22 Microarray Specificity Validated Antibody clone BLCAM/1795. Strong HRP-DAB brown membranous and cytoplasmic staining is observed in B cell-rich regions, highlighting dense populations of lymphocytes consistent with CD22 expression in mature B cells, while surrounding non-lymphoid tissue remains largely negative. The staining pattern supports use of this CD22 antibody for immunohistochemistry-based identification of B cell populations with high specificity. Heat-induced epitope retrieval was performed using citrate buffer (pH 6) for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

CD22 Antibody Spleen IHC. Immunohistochemistry analysis of CD22/Siglec-2 expression in FFPE human spleen tissue using CD22 Microarray Specificity Validated Antibody clone BLCAM/1795. Strong HRP-DAB brown staining is observed in lymphoid follicles within the white pulp, highlighting dense aggregates of B lymphocytes with predominant membranous and cytoplasmic localization, while surrounding red pulp regions and non-lymphoid cells show minimal staining. The distribution pattern is consistent with CD22 expression in mature B cell compartments and supports use of this CD22 antibody for immunohistochemistry-based evaluation of lymphoid tissue architecture with high specificity. Heat-induced epitope retrieval was performed using citrate buffer (pH 6) for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

Western blot testing of FFPE human 1) Raji and 2) Ramos cell lysate with CD22 antibody (clone BLCAM/1795). Expected molecular weight: 76/95 kDa (alpha/beta, unmodified), 130-150 kDa (glycosylated).

CD22 Antibody Specificity Validation. Protein microarray specificity analysis of CD22/Siglec-2 using CD22 Microarray Specificity Validated Antibody clone BLCAM/1795 on a HuProt array containing over 19,000 full-length human proteins. The antibody demonstrates highly selective binding to CD22 with minimal off-target interaction, supporting its specificity for accurate target detection. Z-score represents the signal intensity generated by antibody binding relative to the mean signal across the array, expressed as standard deviations above background. S-score reflects the difference between consecutive ranked Z-scores and indicates the relative specificity of the antibody for its intended target compared to other proteins on the array. These results support the use of this CD22 antibody for applications requiring high specificity and confident target identification.

SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone BLCAM/1795) as confirmation of integrity and purity.