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Home >> Antibodies >> CD22 Antibody

CD22 Antibody [clone BLCAM/1795] (V3693)

  Catalog No Formulation Size Price (USD)  
Image V3693-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3693-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3693SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC testing of FFPE human tonsil tissue with CD22 antibody (clone BLCAM/1795). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human spleen tissue with CD22 antibody (clone BLCAM/1795). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of FFPE human 1) Raji and 2) Ramos cell lysate with CD22 antibody (clone BLCAM/1795). Expected molecular weight: 76/95 kDa (alpha/beta, unmodified), 130-150 kDa (glycosylated).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD22 antibody (clone BLCAM/1795). These results demonstrate the foremost specificity of the BLCAM/1795 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone BLCAM/1795) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name BLCAM/1795
Purity Protein G affinity chromatography
UniProt P20273
Localization Cell surface, cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Flow cytometry : 1-2ug/10^6 cells
Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CD22 antibody is available for research use only.
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Description

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Application Notes

Optimal dilution of the CD22 antibody should be determined by the researcher.

Immunogen

Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.

Storage

Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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