CD22 Antibody / B Cell Internalization and Targeting Antibody [clone CBO-3] (RQ8901)

CD22 Antibody Spleen Tissue IHC Staining. Immunohistochemistry analysis of CD22/Siglec-2 expression in FFPE human spleen tissue using CD22 Antibody / B Cell Internalization and Targeting Antibody. HRP-DAB brown staining is concentrated within lymphoid follicles of the white pulp, highlighting dense populations of B lymphocytes with prominent membranous and cytoplasmic localization, while surrounding red pulp regions and non-lymphoid elements remain largely negative. The staining pattern is consistent with CD22 surface expression and its role in receptor internalization and cellular targeting within B cell compartments. Detection was performed using an HRP-conjugated secondary antibody with DAB substrate. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

CD22 Antibody for WB. Western blot analysis of CD22/Siglec-2 expression using CD22 Antibody / B Cell Internalization and Targeting Antibody in human B cell lymphoma lysates. Lane 1: Raji lysate, Lane 2: Ramos lysate. A band is detected at approximately 130-150 kDa, consistent with the predicted molecular weight of CD22 / CD22 and reflecting its glycosylated form, while potential lower molecular weight species may represent less modified forms of the protein. The banding pattern aligns with the known expression of CD22 in B cell-derived lines and supports its use for western blot analysis of receptor expression and internalization-associated protein processing.