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Home >> Antibodies >> CD21 Antibody

CD21 Antibody [clone CR2/8880R] (V4781)

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  Catalog No Formulation Size Price (USD)  
Image V4781-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4781-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4781SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with CD21 antibody (clone CR2/8880R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD21 antibody (clone CR2/8880R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD21 antibody (clone CR2/8880R) as confirmation of integrity and purity.
CD21 Antibody Raji WB. Western blot analysis of Complement Receptor 2 / CD21 in human Raji cell lysate using recombinant rabbit monoclonal CD21 antibody, clone CR2/8880R. A band is detected at approximately 140-150 kDa, consistent with the predicted molecular weight of full-length CD21 and reflecting its expression in B-cell lymphoma cells.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CR2/8880R
Purity Protein A/G affinity
UniProt P20023
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Blot : 2-4ug/ml
Limitations This CD21 antibody is available for research use only.
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Description

CD21 also known as complement C3d receptor, Epstein-Barr virus receptor, and complement receptor type 2 (CR2), is a protein that in humans is encoded by the CR2 gene. This protein is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. B cells have CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. It is expressed by follicular dendritic cells (FDC) and mature B cells, as well as by several types of epithelial cells. CD21 is useful in the identification of follicular dendritic cell matrix found in normal lymph node and tonsillar tissue, and also useful in identifying abnormal.

For a validated reference of CD21 expression across B cells and follicular dendritic cell networks, see CD21 antibody clone CR2/2754 with supporting multi-application data.

Application Notes

Optimal dilution of the CD21 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD21 antibody.

Storage

Aliquot the CD21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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