CD202b Antibody / TIE2 [clone TEK/9411] (V6009)

Immunohistochemistry analysis of CD202b / TIE2 antibody in human renal cell carcinoma tissue (clone TEK/9411). FFPE human renal cell carcinoma sections show HRP-DAB brown membranous and cytoplasmic staining outlining vascular endothelial cells within tumor-associated microvessels, consistent with TEK receptor tyrosine kinase localization. Tumor epithelial cells demonstrate minimal to weak staining compared to endothelial structures. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, confirming absence of specific brown signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Immunohistochemistry analysis of CD202b / TIE2 antibody in human testis tissue (clone TEK/9411). FFPE human testis sections demonstrate HRP-DAB brown cytoplasmic and membranous staining in cells within seminiferous tubules, consistent with TEK receptor tyrosine kinase expression in vascular and interstitial compartments. Endothelial cells associated with microvasculature show distinct membranous brown chromogenic signal, while surrounding stromal elements display minimal staining. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, confirming absence of specific brown signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

SDS-PAGE Analysis of CD202b/TIE2 antibody (TEK/9411). Confirmation of Purity and Integrity of Antibody.