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Home >> Antibodies >> Cd20 Antibody

Cd20 Antibody (RQ6329)

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  Catalog No Formulation Size Price (USD)  
Image RQ6329 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse spleen tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse spleen tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestinal tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse spleen tissue lysate with Cd20 antibody. Expected molecular weight ~33 kDa but may be observed at up to ~50 kDa due to glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with Cd20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd20 antibody.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P11836
Localization Cell surface, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Cd20 antibody is available for research use only.
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Description

CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What�s more, this protein may be involved in the regulation of B-cell activation and proliferation.

Application Notes

Optimal dilution of the Cd20 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids M1-P291) was used as the immunogen for the Cd20 antibody.

Storage

After reconstitution, the Cd20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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