• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> CD20 Antibody Rabbit Polyclonal

CD20 Antibody Rabbit Polyclonal (RQ6329)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ6329 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
Bulk quote request
›
Immunohistochemistry analysis of CD20 Antibody Rabbit Polyclonal in mouse spleen tissue. FFPE mouse spleen demonstrates membranous HRP-DAB brown staining in scattered lymphoid cells within splenic white pulp regions, consistent with Cd20 expression in B lymphocytes, while surrounding red pulp areas show minimal staining. Antigen retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with Cd20 antibody.
IHC staining of FFPE mouse intestinal tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse spleen tissue lysate with Cd20 antibody. Expected molecular weight ~33 kDa but may be observed at up to ~50 kDa due to glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with Cd20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd20 antibody.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P11836
Localization Cell surface, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Cd20 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : IF, FACS, IHC-P, WB
    Reactivity : Human
    Recmousemono
    Microvalidated
  • Applications : FACS, IHC-P
    Reactivity : Human
    Recrabbitmono
  • Applications : IHC-P, FACS
    Reactivity : Human
    Recrabbitmono
  • Applications : WB, IHC
    Reactivity : Human
    Recrabbitmono
  • Applications : Functional studies, FACS, IF
    Reactivity : Human
    Citation  Citations(5)
  • Applications : FACS, IF
    Reactivity : Human. Other species not known.
    Citation  Citations(5)
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
  • Applications : Functional studies, FACS, IF
    Reactivity : Human
    Citation  Citations(6)
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
  • Applications : FACS, IF, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human
  • Applications : WB, IHC, ELISA (peptide)
    Reactivity : Human
  • Applications : WB, IHC, ELISA (peptide)
    Reactivity : Human
  • Applications : IHC-P
    Reactivity : Human

Description

CD20 antibody recognizes CD20, also known as Membrane spanning 4-domains subfamily A member 1, a B-cell specific transmembrane phosphoprotein encoded by the MS4A1 gene. CD20 is localized to the plasma membrane of B lymphocytes, where it regulates B-cell activation, proliferation, and calcium signaling. CD20 Antibody Rabbit Polyclonal is developed to detect CD20 expression in research applications focused on B-cell biology, lymphoma investigation, and immune system studies.

The MS4A1 gene is located on chromosome 11q12.2 and encodes a protein characterized by four transmembrane domains with short cytoplasmic N- and C-terminal regions. CD20 expression begins at the late pre-B-cell stage and continues through mature B cells but is absent on hematopoietic stem cells and terminally differentiated plasma cells. This restricted lineage expression makes CD20 one of the most widely used markers for identifying B-cell populations in lymphoid tissues such as tonsil, lymph node, spleen, and bone marrow.

Functionally, CD20 associates with the B-cell receptor signaling complex and contributes to calcium influx following antigen engagement. Although CD20 lacks a defined soluble ligand, it participates in membrane microdomains that regulate signal transduction and B-cell activation. Aberrant CD20 expression is characteristic of B-cell non-Hodgkin lymphoma, diffuse large B-cell lymphoma, follicular lymphoma, and chronic lymphocytic leukemia. CD20 Antibody Rabbit Polyclonal supports evaluation of CD20 distribution and density in both normal and malignant B-cell populations.

CD20 is also a clinically significant therapeutic target. Anti-CD20 monoclonal antibodies are widely used to induce B-cell depletion through complement activation, antibody-dependent cellular cytotoxicity, and apoptotic signaling. Investigation of CD20 expression levels and antigen modulation remains important for understanding treatment response and resistance mechanisms.

This rabbit polyclonal antibody recognizes multiple epitopes on the CD20 protein, which can enhance detection sensitivity and support identification of native and denatured forms. CD20 Antibody Rabbit Polyclonal provides a dependable reagent for studying B-cell development, lymphoma biology, and immune regulation in translational and basic research settings.

Application Notes

Optimal dilution of the Cd20 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids M1-P291) was used as the immunogen for the Cd20 antibody.

Storage

After reconstitution, the Cd20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all CD20 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.