CD20 Antibody Protein Microarray Validated [clone MS4A1/4655] (V9456)

Western blot testing of human Raji cell lysate with CD20 antibody (clone MS4A1/4655). Predicted molecular weight ~33 kDa.

Immunohistochemistry analysis of CD20 Antibody Protein Microarray Validated in human tonsil tissue. FFPE human tonsil demonstrates strong membranous HRP-DAB brown staining in B lymphocytes within germinal centers and follicular regions, consistent with CD20/MS4A1 surface expression, while adjacent non-B-cell areas show minimal staining. Antigen retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to incubation with CD20 antibody (clone MS4A1/4655).

IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/4655). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/4655) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD20 antibody (clone MS4A1/4655). These results demonstrate the foremost specificity of the MS4A1/4655 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.