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Home >> Antibodies >> CD20 Antibody

CD20 Antibody [clone MS4A1/4655] (V9456)

  Catalog No Formulation Size Price (USD)  
Image V9456-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9456-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9456SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Western blot testing of human Raji cell lysate with CD20 antibody (clone MS4A1/4655). Predicted molecular weight ~33 kDa.
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/4655). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/4655). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/4655) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD20 antibody (clone MS4A1/4655). These results demonstrate the foremost specificity of the MS4A1/4655 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human, Mouse and Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MS4A1/4655
Purity Protein A affinity
UniProt P11836
Localization Cell Surface
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD20 antibody is available for research use only.
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Description

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Application Notes

Optimal dilution of the CD20 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 266-297 from the cytoplasmic domain was used as the immunogen for the CD20 antibody.

Storage

Aliquot the CD20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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