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Home >> Antibodies >> CD20 Antibody

CD20 Antibody [clone MS4A1/3411] (V7618)

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  Catalog No Formulation Size Price (USD)  
Image V7618-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7618-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7618SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7618IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).
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Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3411). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human Raji cells with CD20 antibody (clone MS4A1/3411); Red=isotype control, Blue= CD20 antibody.
Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3411). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3411) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name MS4A1/3411
Purity Protein G affinity chromatography
UniProt P11836
Localization Predominantly cell surface with some cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This CD20 antibody is available for research use only.
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Description

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 213-297 from the human protein were used as the immunogen for this CD20 antibody.

Storage

CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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