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Home >> Antibodies >> CD2 Antibody

CD2 Antibody [clone HuLy-m1] (V2951)

  Catalog No Formulation Size Price (USD)  
Image V2951-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2951-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2951SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
Western blot testing of Jurkat cell lysate with CD2 antibody (clone HuLy-m1). Expected molecular weight ~47kDa.
Flow cytometry testing of human MOLT-4 cells with CD2 antibody (clone HuLy-m1); Red=isotype control, Blue= CD2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name HuLy-m1
Purity Protein G affinity chromatography
UniProt P06729
Localization Cell surface
Applications Functional studies (order BSA/sodium azide-free format) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD2 antibody is available for research use only.
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Description

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.

Application Notes

Optimal dilution of the CD2 antibody should be determined by the researcher.

Immunogen

Human thymocytes were used as the immunogen for the CD2 antibody.

Storage

Store the CD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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