CD19 Molecule Antibody [clone CD19/4260R] (V6034)

Western blot analysis of CD19/CD19 molecule antibody in human tonsil tissue lysate. A strong band is detected at approximately 95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19), a glycosylated B cell surface receptor. The observed band migrates slightly above the core protein size, which is expected due to N-linked glycosylation of CD19. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R).

Western blot analysis of CD19/CD19 molecule antibody in human Raji cell lysate. A prominent band is detected at approximately 95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19), a glycosylated B cell surface receptor expressed in B cell lines such as Raji. The apparent molecular weight above the core protein size reflects known N-linked glycosylation of CD19. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R).

Western blot analysis of CD19/CD19 molecule antibody in human Raji cell lysate. A distinct band is detected at approximately 90-95 kDa, consistent with the predicted molecular weight of CD19 (Cluster of Differentiation 19). The slightly diffuse migration pattern is expected, as CD19 is a glycosylated B cell surface receptor and N-linked glycosylation contributes to an increased apparent molecular weight on SDS-PAGE. Blot was probed with CD19/CD19 molecule antibody (clone CD19/4260R7).

Immunohistochemistry analysis of CD19/CD19 molecule antibody in human tonsil tissue. FFPE human tonsil demonstrates strong membranous HRP-DAB brown staining in B lymphocytes within germinal centers, consistent with CD19 (Cluster of Differentiation 19) expression on mature B cells, while surrounding interfollicular T cell-rich areas show minimal staining. The inset image using PBS in place of primary antibody as a secondary-only negative control shows absence of specific signal. Antigen retrieval was performed by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to incubation with CD19/CD19 molecule antibody (clone CD19/4260R).

Protein microarray specificity analysis of CD19/CD19 molecule antibody using a HuProt full-length human protein array containing more than 19,000 recombinant human proteins. CD19 Recombinant Rabbit Monoclonal Antibody (clone CD19/4260R) demonstrates the strongest signal for CD19 with a Z-score of 102.09 and an S-score of 85.25, indicating high affinity and target specificity. The next highest ranked proteins, including GPR183 and IST1, show substantially lower Z- and S-scores, supporting selective binding to CD19.
The Z-score represents the strength of the fluorescent signal generated when the monoclonal antibody, in combination with a fluorescently labeled anti-IgG secondary antibody, binds to a protein on the HuProt array. Z-scores are expressed as standard deviations above the mean signal of all proteins on the array. When targets are ranked in descending order by Z-score, the S-score represents the difference in standard deviations between the top-ranked target and the next highest binding protein. An S-score of at least 2.5 is generally considered indicative of target specificity. The high S-score observed for CD19 relative to other proteins confirms strong specificity of CD19/CD19 molecule antibody (clone CD19/4260R) for its intended target.

SDS-PAGE Analysis of Purified CD19/CD19 molecule antibody (clone CD19/4260R). Confirmation of Purity and Integrity of Antibody.