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Home >> Antibodies >> CD19 Antibody

CD19 Antibody [clone AOFO-3] (RQ5485)

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  Catalog No Formulation Size Price (USD)  
Image RQ5485 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
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Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
Western blot testing of human 1) Raji and 2) Daudi cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
IHC staining of FFPE human Hodgkin's lymphoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal carcinoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse skin tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat stomach tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AOFO-3
Purity Affinity purified
UniProt P15391
Localization Cell surface, cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This CD19 antibody is available for research use only.
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Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [RefSeq]

Application Notes

Optimal dilution of the CD19 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human CD19 was used as the immunogen for the CD19 antibody.

Storage

Store the CD19 antibody at -20oC.

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