- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD19 Antibody for FACS [clone PDR134] (V8223)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V8223-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V8223-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V8223SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgM, kappa |
| Clone Name | PDR134 |
| Purity | PEG precipitation |
| UniProt | P15391 |
| Localization | Cell surface, cytoplasmic |
| Applications | Flow Cytometry : 1-2ug/10^6 cells in 0.1ml Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml |
| Limitations | This CD19 antibody is available for research use only. |
|
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CD19 antibody recognizes CD19, a B cell-specific type I transmembrane glycoprotein that functions as a central coreceptor in B cell receptor signaling. CD19 Antibody for FACS (Clone PDR134) is developed for reliable detection of CD19 on the surface of B lymphocytes in flow cytometry-based applications. CD19 is a member of the immunoglobulin superfamily and is expressed from early pro-B cell stages through mature peripheral B lymphocytes, where it localizes to the plasma membrane. By forming a signaling complex with CD21 and CD81, CD19 amplifies antigen receptor-mediated signaling and lowers the activation threshold required for B cell activation.
The CD19 gene is located on chromosome 16p11.2 and encodes a protein containing two extracellular immunoglobulin-like domains, a single transmembrane region, and a cytoplasmic tail enriched in tyrosine residues that become phosphorylated upon B cell stimulation. These phosphorylation events recruit signaling molecules such as PI3K and adaptor proteins, driving downstream pathways that regulate B cell proliferation, differentiation, and survival. CD19 Antibody for FACS (Clone PDR134) supports investigations into B cell development, activation status, and immune profiling in both normal and disease contexts.
In normal tissues and peripheral blood, CD19 expression is restricted to B lineage cells within bone marrow, lymph node, tonsil, spleen, and circulating B cells. Plasma cells typically show reduced or absent CD19 expression, reflecting terminal differentiation. Because of its lineage specificity and stable surface expression, CD19 serves as a reliable pan-B cell marker for flow cytometric identification and characterization of B cell subsets.
Aberrant CD19 expression is a defining feature of most B cell malignancies, including B cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and various non-Hodgkin lymphomas. CD19 is also a major therapeutic target in immunotherapy strategies such as chimeric antigen receptor T cell approaches. Accurate detection of CD19-positive cells is therefore critical for studies of tumor immunophenotyping, immune monitoring, and therapeutic development.
Clone PDR134 is a monoclonal antibody designed to provide specific and reproducible detection of CD19 in flow cytometry experiments. CD19 Antibody for FACS (Clone PDR134) enables clear discrimination of CD19-positive B cells from other leukocyte populations, supporting precise immunophenotypic analysis and B cell-focused research applications.
Optimal dilution of the CD19 antibody should be determined by the researcher.
Pokeweek-stimulated Daudi and Raji cells were used as the immunogen for the CD19 for FACS antibody.
Store the CD19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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