CD19 Antibody Protein Microarray Validated [clone CD19/7665] (V4404)

Immunohistochemistry analysis of CD19 Antibody Protein Microarray Validated in human tonsil tissue. FFPE human tonsil shows strong membranous HRP-DAB brown staining in B lymphocytes within lymphoid follicles, consistent with CD19 (Cluster of Differentiation 19) expression on mature B cells. Antigen retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to incubation with CD19 antibody (clone CD19/7665).

IHC staining of FFPE human tonsil tissue with CD19 antibody (clone CD19/7665). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD19 antibody (clone CD19/7665). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.