CD19 Antibody Mouse Monoclonal [clone CD19/3116] (V8224)

Immunohistochemistry analysis of CD19 Antibody Mouse Monoclonal in human tonsil tissue. FFPE human tonsil demonstrates strong membranous HRP-DAB brown staining in B lymphocytes within germinal centers and follicular regions, consistent with CD19 (Cluster of Differentiation 19) expression on mature B cells, while interfollicular T cell-rich areas show reduced staining. Antigen retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 minutes followed by cooling prior to incubation with CD19 antibody (clone CD19/3116).

IHC staining of FFPE human tonsil with CD19 antibody (clone CD19/3116). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of human Raji cells with CD19 antibody (clone CD19/3116); Red=isotype control, Blue= CD19 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD19 antibody (clone CD19/3116). These results demonstrate the foremost specificity of the CD19/3116 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD19 antibody (clone CD19/3116) as confirmation of integrity and purity.