CD171 Antibody / L1 Cell Adhesion Molecule Antibody (RQ7089)

CD171 Antibody Mouse Brain Neuronal Tissue IHC. Immunohistochemistry staining of paraffin-embedded mouse brain tissue with CD171 Antibody / L1 Cell Adhesion Molecule Antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in EDTA buffer, pH 8. Tissue sections were blocked with 10% goat serum and incubated with primary antibody overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. CD171 staining is observed in neuronal populations within the mouse brain tissue.

CD171 Antibody Rat Brain Neuronal Tissue IHC. Immunohistochemistry staining of paraffin-embedded rat brain tissue with CD171 Antibody / L1 Cell Adhesion Molecule Antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in EDTA buffer, pH 8. Tissue sections were blocked with 10% goat serum and incubated with primary antibody overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. CD171 staining is observed in neuronal populations within the rat brain tissue.

CD171 Antibody Human, Mouse and Rat WB. Western blot analysis of human HeLa whole cell lysate, rat brain and mouse brain tissue lysates using CD171 Antibody / L1 Cell Adhesion Molecule Antibody at 0.5 ug/ml. Samples were separated by 10% SDS-PAGE under reducing conditions and transferred to nitrocellulose. L1CAM is detected at approximately 220 kDa, compared with a predicted molecular weight of approximately 140 kDa. The higher apparent molecular weight is consistent with extensive glycosylation of L1CAM. Additional lower molecular weight bands may reflect proteolytic processing of the protein.

CD171 Antibody Human Caco-2 Colorectal Cancer Cell FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 colorectal cancer cells using CD171 Antibody / L1 Cell Adhesion Molecule Antibody at 1 ug/1x10^6 cells. Cells were blocked with 10% normal goat serum and incubated with primary antibody for 30 min at 20oC, followed by DyLight 488-conjugated goat anti-rabbit IgG. The blue histogram represents CD171 antibody staining, green represents the isotype control, and red represents the blank control. The strong fluorescence shift demonstrates detection of CD171 in Caco-2 cells by flow cytometry.