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Home >> Antibodies >> CD168 Antibody / RHAMM Antibody

CD168 Antibody / RHAMM Antibody (F54611)

  Catalog No Formulation Size Price (USD)  
Image F54611-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F54611-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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CD168 Antibody Staining of Human Breast Carcinoma IHC. Immunohistochemistry staining of FFPE human breast carcinoma tissue using CD168 Antibody. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling. Brown immunoreactivity is evident throughout the carcinoma cells, with predominantly cytoplasmic staining.
CD168 Antibody Detection in Human Leukemia and Prostate Cancer Cells WB. Western blot analysis of human K562 chronic myelogenous leukemia (lane 1), LNCaP prostate cancer (lane 2) and HL-60 acute promyelocytic leukemia (lane 3) cell lysates using CD168 Antibody. A prominent band is detected at approximately 80-85 kDa across the tested lysates, consistent with the reported intracellular form of HMMR/RHAMM.
CD168 Antibody Detection in Ramos Burkitt Lymphoma Cells WB. Western blot analysis of human Ramos Burkitt lymphoma cell lysate using CD168 Antibody. A distinct band is detected at approximately 80 kDa, consistent with the reported intracellular form of HMMR/RHAMM.
CD168 Antibody Detection in Ramos Burkitt Lymphoma Cells FACS. Flow cytometry analysis of fixed and permeabilized human Ramos Burkitt lymphoma cells using CD168 Antibody. The antibody-stained population (green) shows a pronounced rightward fluorescence shift relative to the isotype control (blue), demonstrating CD168/RHAMM detection under the tested conditions.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O75330
Localization Cell surface, cytoplasmic
Applications Western Blot : 1:500-1:2000
Flow Cytometry : 1:25 (million cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This CD168 Antibody / RHAMM Antibody is available for research use only.
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Description

CD168 Antibody / RHAMM Antibody recognizes CD168, a multifunctional hyaluronan-associated protein encoded by the HMMR gene. Also widely known as RHAMM, the receptor for hyaluronan-mediated motility, this protein participates in cell migration, cytoskeletal organization and cell-cycle regulation. HMMR can occupy extracellular, cell-associated and intracellular locations depending on cellular context, allowing it to participate in distinct mechanisms involving hyaluronan signaling and cytoskeletal dynamics.

Intracellular CD168 associates with microtubules, centrosomes and mitotic spindle structures. These interactions contribute to spindle organization, chromosome segregation and progression through mitosis. RHAMM also interacts with proteins involved in microtubule organization and motor activity, connecting its function to the dynamic cytoskeletal rearrangements required during cell division.

CD168 has additionally been associated with hyaluronan-dependent cell motility and growth factor signaling. Interactions involving hyaluronan, cytoskeletal components and MAPK pathway proteins can influence cellular migration and proliferation. A RHAMM Antibody can therefore support research into mechanisms linking extracellular matrix signals with intracellular cytoskeletal organization and proliferative responses.

CD168 is extensively investigated in cancer biology. Increased HMMR expression has been reported in multiple malignancies and has been associated with tumor-cell proliferation, invasion and disease progression. Its combined functions in mitosis, migration and cytoskeletal regulation provide several mechanisms through which altered CD168 expression may contribute to malignant cell behavior.

This rabbit polyclonal CD168 Antibody was generated against a C-terminal region spanning amino acids 668-697 of human HMMR and is antigen affinity purified. For a broadly targeted reagent and our primary HMMR resource, see our HMMR Antibody / Hyaluronan-Mediated Motility Receptor Antibody page. NSJ Bioreagents provides antibodies for research into cell motility, mitosis and cancer biology. A CD168 Antibody provides a research tool for investigating RHAMM/HMMR and its roles in cytoskeletal regulation, cell migration and tumor biology.

For a broadly targeted reagent and our primary HMMR resource, see our HMMR Antibody / Hyaluronan-Mediated Motility Receptor Antibody page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the CD168 Antibody / RHAMM Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 668-697 from the human protein was used as the immunogen for the CD168 antibody.

Storage

Aliquot the CD168 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

RHAMM antibody, HMMR antibody, Receptor for hyaluronan-mediated motility antibody, Hyaluronan-mediated motility receptor antibody, IHABP antibody

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