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Home >> Antibodies >> CD168 Antibody / HMMR

CD168 Antibody / HMMR (F54611)

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  Catalog No Formulation Size Price (USD)  
Image F54611-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54611-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human breast carcinoma tissue with CD168 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human breast carcinoma tissue with CD168 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) K562, 2) LNCaP and 3) HL60 cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
Western blot testing of human Ramos cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
Flow cytometry testing of human Ramos cells with CD168 antibody; Blue=isotype control, Green= CD168 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O75330
Localization Cell surface, cytoplasmic
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This CD168 antibody is available for research use only.
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Description

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Application Notes

The stated application concentrations are suggested starting points. Titration of the CD168 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 668-697 from the human protein was used as the immunogen for the CD168 antibody.

Storage

Aliquot the CD168 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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