CD161 Antibody KLRB1/8910 / KLRB1 Monoclonal Antibody [clone KLRB1/8910] (V5592)

CD161 Antibody KLRB1/8910 by WB in Human Spleen. Western blot analysis of human spleen tissue lysate using CD161 Antibody KLRB1/8910. A prominent immunoreactive band is observed at approximately 25 kDa, consistent with the predicted molecular weight of CD161/KLRB1. A higher molecular weight band is also visible near 200 kDa, although its identity has not been established. CD161 is a type II transmembrane glycoprotein expressed on natural killer cells and subsets of T lymphocytes. Glycosylation can influence its apparent molecular weight during electrophoresis.

CD161 Antibody KLRB1/8910 by IHC in Human Prostate. Immunohistochemistry of FFPE human prostate tissue using CD161 Antibody KLRB1/8910. Strong brown DAB staining is observed in the glandular epithelium, with prominent cytoplasmic immunoreactivity in epithelial cells. Surrounding stromal tissue exhibits comparatively weaker staining, while nuclei are counterstained blue with hematoxylin. The inset shows a negative control in which PBS replaced the primary antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

CD161 Antibody KLRB1/8910 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CD161 Antibody KLRB1/8910 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. Under non-reducing conditions, the intact IgG migrates at approximately 150 kDa. Under reducing conditions, the antibody separates into heavy and light chains migrating at approximately 50 kDa and 25 kDa, respectively. The observed banding pattern is consistent with the expected molecular structure of an IgG antibody and supports the integrity and purity of the preparation.