CD15 Antibody / Leukocyte Differentiation Antigen Antibody [clone Leu-M1, also called MMA] (V3177)

Description

CD15, also known as Lewis X (Lex), is a carbohydrate adhesion antigen expressed predominantly on granulocytes, subsets of monocytes, and differentiated myeloid-associated cellular populations. CD15 functions in leukocyte adhesion, phagocyte trafficking, inflammatory signaling, and selectin-mediated cell-cell interaction pathways through its role as a fucosylated carbohydrate determinant present on glycoproteins and glycolipids. CD15 Antibody is useful for investigations involving leukocyte differentiation, hematopoietic lineage specification, inflammatory signaling pathways, and hematopathology-associated cellular characterization.

CD15 antibody, also referred to as Lewis X antibody, Leu-M1 antibody, and MMA antibody in the literature, recognizes a carbohydrate epitope generated through fucosyltransferase-associated glycosylation pathways including FUT4-mediated biosynthesis. CD15 expression is strongly associated with mature granulocytes, myeloid differentiation, subsets of activated immune cells, and characteristic Reed-Sternberg cell populations in classical Hodgkin lymphoma. The antigen localizes predominantly to the cell membrane and glycoconjugate-rich cellular surfaces involved in leukocyte adhesion and immune-associated signaling pathways.

CD15 Antibody / Leukocyte Differentiation Antigen Antibody (clone Leu-M1/MMA) is uniquely positioned for studies involving immune differentiation, hematopoietic lineage characterization, and tumor-associated leukocyte marker expression. This mouse monoclonal antibody has been referenced in more than 1700 publications and supports immunohistochemistry, immunofluorescence, and flow cytometry applications involving granulocytic, myeloid, and Hodgkin lymphoma-associated cellular populations. The extensive literature history associated with clones Leu-M1 and MMA supports broad use in investigations involving hematopathology, leukocyte differentiation, and immune-associated cellular phenotyping.

CD15-associated carbohydrate structures contribute to leukocyte rolling, endothelial adhesion, inflammatory recruitment, and selectin-mediated trafficking pathways during immune activation. Altered CD15 expression patterns have been associated with myeloid maturation status, inflammatory responses, hematologic malignancies, and epithelial tumor-associated glycosylation changes. Because CD15 expression is strongly associated with differentiated granulocytic cellular populations and Reed-Sternberg cells, it has become an important marker in studies involving hematopoietic differentiation and lymphoma-associated immunophenotyping.

In tissue-based detection systems, CD15 expression commonly demonstrates membranous and cytoplasmic staining patterns within granulocytes, inflammatory infiltrates, and Hodgkin lymphoma-associated neoplastic cellular populations. Immunofluorescence and flow cytometry applications additionally support characterization of immune-associated cell surface expression and leukocyte differentiation status in suspension cell populations. The Leu-M1 clone designation is historically well established in hematopathology and lymphoma-associated diagnostic investigations involving Reed-Sternberg cell identification and granulocyte-associated lineage characterization.

This CD15 Antibody supports research involving leukocyte differentiation, hematopoietic lineage biology, inflammatory signaling pathways, granulocyte maturation, Hodgkin lymphoma-associated immunophenotyping, selectin-mediated adhesion biology, and immune-associated glycosylation regulation. Clone Leu-M1/MMA may be incorporated into immunohistochemistry, immunofluorescence, flow cytometry, and tissue-based investigations examining immune differentiation and hematopathology-associated cellular organization in normal and diseased tissues.

For additional hematopoietic differentiation and immune lineage markers, explore our Cancer Antibodies page featuring antibodies targeting leukocyte-associated signaling, hematopathology, and lymphoma differentiation pathways.

Application Notes

The optimal dilution of the CD15 Antibody / Leukocyte Differentiation Antigen Antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The histiocytic cell line U937 was used as the immunogen for this CD15 antibody.

Storage

Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

CD15 antibody, Lewis X antibody, Leu-M1 antibody, MMA antibody, FUT4 carbohydrate antigen antibody