CD146 Rodent Antibody / MCAM Polyclonal Antibody (RQ5987)

CD146 Rodent Antibody by IF in Mouse Intestine Tissue. Immunofluorescence staining of FFPE mouse intestine tissue using rabbit polyclonal CD146 antibody. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer (pH 8.0) for 20 minutes. Green fluorescence demonstrates MCAM immunoreactivity in vascular and stromal structures surrounding the intestinal glands, with comparatively weak staining in much of the glandular epithelium. Cell nuclei are counterstained blue with DAPI.

CD146 Rodent Antibody by IHC in Mouse Intestine Tissue. Immunohistochemistry staining of FFPE mouse intestine tissue using rabbit polyclonal CD146 antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Brown immunoreactivity is observed in stromal and vascular-associated cells surrounding the intestinal glands, with staining concentrated along cellular boundaries and within the connective tissue between glandular structures. Nuclei are counterstained blue.

CD146 Rodent Antibody by IHC in Mouse Intestine Tissue. Immunohistochemistry staining of FFPE mouse intestine tissue using rabbit polyclonal CD146 antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Brown MCAM immunoreactivity is distributed throughout stromal regions surrounding the intestinal glands, with prominent staining along elongated vascular and connective tissue structures. Much of the glandular epithelium exhibits comparatively weaker staining, and nuclei are counterstained blue.

CD146 Rodent Antibody by IHC in Rat Spleen Tissue. Immunohistochemistry staining of FFPE rat spleen tissue using rabbit polyclonal CD146 antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling. Scattered brown immunoreactivity is observed in vascular-associated structures and selected stromal cells, while much of the surrounding splenic tissue exhibits relatively weak staining. Nuclei are counterstained blue.

CD146 Rodent Antibody by WB in Rat and Mouse Tissue and Cell Lysates. Western blot analysis of rat lung (lane 1), rat ovary (lane 2), rat PC-12 cells (lane 3), mouse lung (lane 4), mouse spleen (lane 5), mouse thymus (lane 6), mouse kidney (lane 7), and mouse ovary (lane 8) using rabbit polyclonal CD146 antibody. Strong immunoreactive bands are detected at approximately 120-130 kDa in lanes 1-3 and 8, with weaker bands at similar molecular weights in lanes 4-7. These bands are consistent with the expected migration range of glycosylated MCAM, a cell surface adhesion molecule with an apparent molecular weight that varies according to glycosylation.

CD146 Rodent Antibody by FACS in Mouse Neuro-2a Cells. Flow cytometry analysis of mouse Neuro-2a cells using rabbit polyclonal CD146 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares antibody-stained cells (blue), isotype control (green), and unstained cells (red). The antibody-stained population exhibits a distinct rightward fluorescence shift relative to both controls, demonstrating detection of MCAM/CD146 in Neuro-2a cells.

CD146 Rodent Antibody by FACS in Rat C6 Cells. Flow cytometry analysis of rat C6 cells using rabbit polyclonal CD146 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares antibody-stained cells (blue), isotype control (green), and unstained cells (red). A clear rightward fluorescence shift is observed in the antibody-stained population compared with both controls, demonstrating detection of MCAM/CD146 in rat C6 cells.