CD141 Antibody / BDCA3 Dendritic Cell Marker [clone COO-20] (RQ5173)

CD141 Antibody / BDCA3 Dendritic Cell Marker immunoblotting. Western blot analysis of Thrombomodulin / CD141 (THBD) expression using CD141 Antibody in human placenta lysate. Lane 1: human placenta lysate. A band is detected at approximately 100 kDa, consistent with the predicted molecular weight of Thrombomodulin / CD141 and the known glycosylated form of this membrane receptor. The predicted molecular weight of the core protein is approximately 60 kDa, while extensive glycosylation commonly produces a higher apparent molecular weight band between ~90-100 kDa in SDS-PAGE.

CD141 Antibody / BDCA3 Dendritic Cell Marker. Immunohistochemistry analysis of Thrombomodulin / CD141 (THBD) expression in formalin-fixed, paraffin-embedded human bladder tissue. Using the CD141 Antibody / BDCA3 Dendritic Cell Marker, membranous HRP-DAB brown staining highlights scattered CD141-positive cells with dendritic morphology within the stromal compartment, consistent with BDCA3-positive dendritic cells involved in antigen presentation. Surrounding urothelial cells are largely negative, while occasional vascular endothelial cells also show membranous staining consistent with known thrombomodulin expression. HIER: tissue sections were boiled in pH6, 10mM citrate buffer for 10-20 min followed by cooling prior to staining.