CD14 Polyclonal Antibody / Monocyte Marker Antibody (RQ6836)

CD14 Polyclonal Antibody by IHC in Human Lymphadenoma. Immunohistochemistry staining of FFPE human lymphadenoma tissue using rabbit polyclonal CD14 antibody. Brown immunoreactivity is distributed among numerous cells throughout the tissue, with prominent staining along cell membranes and within the cytoplasm. The positive cells are interspersed with more weakly stained regions, producing a heterogeneous pattern. This distribution is consistent with CD14 expression in monocyte/macrophage populations and supports the use of this Monocyte Marker Antibody for investigating myeloid cells in lymphoid tissues. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

CD14 Polyclonal Antibody by IHC in Human Spleen. Immunohistochemistry staining of FFPE human spleen tissue using rabbit polyclonal CD14 antibody. Scattered brown-positive cells are visible throughout the tissue, with areas of increased staining density surrounding more lightly stained regions. Immunoreactivity is predominantly cytoplasmic and membranous, consistent with CD14 expression in resident monocytes and macrophages. The heterogeneous distribution reflects differences in CD14 expression among splenic cell populations. This experiment demonstrates detection of CD14 in human spleen, where mononuclear phagocytes contribute to immune surveillance and clearance of circulating material. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

CD14 Polyclonal Antibody by IHC in Human Tonsil. Immunohistochemistry staining of FFPE human tonsil tissue using rabbit polyclonal CD14 antibody. Numerous brown-positive cells are concentrated in a broad region of the tissue, while adjacent densely packed lymphoid areas exhibit substantially weaker staining. The positive cells display predominantly membranous and cytoplasmic immunoreactivity, creating a clear contrast with the blue hematoxylin-counterstained lymphocytes. This distribution is consistent with CD14 expression in myeloid cell populations within tonsillar tissue. The image illustrates the utility of this Monocyte Marker Antibody for identifying CD14-positive cells within heterogeneous lymphoid structures. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

CD14 Polyclonal Antibody by IHC in Human Placenta. Immunohistochemistry staining of FFPE human placental tissue using rabbit polyclonal CD14 antibody. Prominent brown immunoreactivity outlines portions of the chorionic villi, with additional staining visible within villous stromal regions. The staining varies between individual villi, producing a heterogeneous pattern against the blue hematoxylin counterstain. CD14 expression in placental tissue is associated with immune cell populations, including macrophages involved in maternal-fetal immune regulation. The image demonstrates antibody reactivity within placental structures, although the staining pattern alone does not establish the identity of individual positive cells. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

CD14 Polyclonal Antibody by IHC in Human Renal Cell Carcinoma. Immunohistochemistry staining of FFPE human renal cell carcinoma tissue using rabbit polyclonal CD14 antibody. Brown immunoreactivity is visible in numerous cells distributed throughout the tumor section, with staining concentrated along cellular borders and within portions of the cytoplasm. The intensity varies among cells, and several regions contain comparatively weakly stained cells. CD14 is associated with monocyte/macrophage populations, including tumor-associated macrophages that participate in inflammatory signaling within the tumor microenvironment. This experiment demonstrates antibody reactivity in renal carcinoma tissue, although the image alone cannot distinguish CD14-positive immune cells from other reactive cell populations. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

CD14 Polyclonal Antibody by IF in Human SiHa Cells. Immunofluorescence staining of FFPE human SiHa cells using rabbit polyclonal CD14 antibody. Green fluorescence is visible predominantly in the cytoplasm and along the periphery of individual cells, surrounding blue DAPI-stained nuclei. The fluorescence intensity varies between cells, with relatively strong staining around several nuclei and less intense staining in other regions. The image demonstrates detectable CD14-associated fluorescence in the tested SiHa cells and supports the use of this Monocyte Marker Antibody for examining cellular CD14 distribution. Heat-induced epitope retrieval was performed by steaming samples in citrate buffer, pH 6, for 20 minutes.

CD14 Polyclonal Antibody by FACS in Human Caco-2 Cells. Flow cytometry analysis of human Caco-2 cells using rabbit polyclonal CD14 antibody at 1 ug per million cells following blocking with goat serum. The antibody-stained population (blue) exhibits a pronounced rightward fluorescence shift relative to the isotype control (green) and unstained cells (red). The blue and green distributions show relatively little overlap, indicating increased antibody-associated fluorescence under the experimental conditions. The histogram demonstrates detection of CD14-associated cell surface staining in Caco-2 cells and provides flow cytometry validation for this antibody. CD14, also known as the lipopolysaccharide receptor, participates in bacterial endotoxin recognition and innate immune signaling.