CD14 Rodent Antibody / Mouse and Rat CD14 Antibody (R32821)

CD14 Rodent Antibody by IHC in Mouse Spleen. Immunohistochemistry staining of FFPE mouse spleen tissue using rabbit polyclonal CD14 antibody at 1 ug/ml. Scattered brown-positive cells are visible predominantly within the splenic red pulp and perifollicular regions, while the densely populated white pulp exhibits comparatively little staining. The positive cells display cytoplasmic and peripheral staining, consistent with CD14 expression in resident myeloid populations. This image demonstrates the utility of a Mouse and Rat CD14 Antibody for identifying monocyte/macrophage-associated cells within lymphoid tissue. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer, pH 6, for 20 minutes.

CD14 Rodent Antibody by IHC in Rat Spleen. Immunohistochemistry staining of FFPE rat spleen tissue using rabbit polyclonal CD14 antibody at 1 ug/ml. Numerous scattered brown-positive cells are distributed throughout the splenic tissue, with areas of increased staining density surrounding more lightly stained lymphoid regions. Immunoreactivity appears predominantly cytoplasmic, with staining extending toward the periphery of individual cells. The heterogeneous distribution is consistent with CD14 expression in splenic monocytes, macrophages, and related myeloid populations. This experiment demonstrates antibody reactivity in rat spleen and complements the corresponding mouse tissue validation. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer, pH 6, for 20 minutes.

CD14 Rodent Antibody by IHC in Rat Lymph Tissue. Immunohistochemistry staining of FFPE rat lymph tissue using rabbit polyclonal CD14 antibody at 1 ug/ml. Brown immunoreactivity highlights scattered individual cells and small groups of cells within the lymphoid tissue, with stronger staining concentrated in selected regions. Most surrounding lymphoid cells exhibit little or no brown staining, providing contrast with the CD14-positive population. The distribution is consistent with the presence of monocyte/macrophage-associated cells within rat lymphoid tissue. This image provides additional tissue validation for the Mouse and Rat CD14 Antibody. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer, pH 6, for 20 minutes.

CD14 Rodent Antibody by WB in Mouse Thymus and Spleen. Western blot analysis of mouse thymus lysate (lane 1) and mouse spleen lysate (lane 2) using rabbit polyclonal CD14 antibody at 0.5 ug/ml. Both samples exhibit prominent immunoreactive bands near 55 kDa, consistent with the expected molecular weight of glycosylated CD14. The bands migrate at similar positions, with the spleen sample displaying slightly greater intensity. Additional higher molecular weight bands are visible near 100 kDa in both lanes. CD14 has a predicted unmodified molecular weight of approximately 40 kDa, while glycosylation can increase its apparent molecular weight to approximately 50-55 kDa. The identity of the higher molecular weight bands has not been established by this experiment.

CD14 Rodent Antibody by FACS in Mouse Peripheral Blood Mononuclear Cells. Flow cytometry analysis of mouse peripheral blood mononuclear cells using rabbit polyclonal CD14 antibody at 1 ug per million cells following blocking with goat serum. The antibody-stained population (blue) exhibits a pronounced rightward fluorescence shift relative to the isotype control (green) and unstained cells (red). The three populations show distinct fluorescence distributions, with the CD14 antibody producing the highest fluorescence intensity. This result demonstrates detectable CD14-associated staining in mouse peripheral blood mononuclear cells and supports the use of this Mouse and Rat CD14 Antibody for flow cytometric analysis of rodent immune cell populations.