Cd14 Antibody (R32821)

Immunohistochemistry analysis of Cd14 antibody in mouse spleen tissue. Formalin-fixed, paraffin-embedded mouse spleen tissue was stained using Cd14 antibody at a concentration of 1 ug per mL. Heat-induced epitope retrieval was performed by steaming tissue sections in citrate buffer, pH 6.0, for 20 minutes, followed by cooling prior to antibody incubation. Brown chromogenic signal is observed in scattered immune cells within splenic red pulp and perifollicular regions, consistent with Cd14-positive myeloid cell populations, while the majority of lymphoid cells in white pulp areas show little to no staining. This staining pattern reflects myeloid lineage-associated expression of Cd14 in mouse spleen tissue.

IHC testing of FFPE rat spleen tissue with Cd14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat lymph tissue with Cd14 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Western blot testing of 1) mouse thymus and 2) mouse spleen with Cd14 antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa (unmodified) and 50-55 kDa (glycosylated).

Flow cytometry testing of mouse PBM cells with Cd14 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd14 antibody.