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Home >> Antibodies >> CD14 Antibody (Macrophage Marker)

CD14 Antibody (Macrophage Marker) [clone LPSR/654] (V2418)

  Catalog No Formulation Size Price (USD)  
Image V2418-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2418-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2418SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name LPSR/654
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P08571
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Limitations This CD14 antibody is available for research use only.
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Description

Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Application Notes

Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.

Immunogen

Human CD14/LPSR recombinant protein was used as immunogen for this CD14 antibody.

Storage

CD14 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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