CD14 Rabbit Polyclonal Antibody / Lipopolysaccharide Receptor Antibody (F49568)

CD14 Rabbit Polyclonal Antibody by IF in Human A549 Cells. Confocal immunofluorescence analysis of human A549 cells using rabbit polyclonal CD14 antibody, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (green). DAPI was used to counterstain nuclei (blue). Green fluorescence is distributed throughout the extranuclear regions of the cells, with a granular cytoplasmic appearance and areas of increased fluorescence intensity. The staining demonstrates detection of CD14, also known as the Lipopolysaccharide Receptor, in A549 cells. The fluorescence pattern is consistent with the reported cellular distribution of CD14, which can be associated with the plasma membrane and intracellular compartments.

CD14 Rabbit Polyclonal Antibody by WB in Transfected Human 293 Cells. Western blot analysis of human 293 cell lysates using rabbit polyclonal CD14 antibody. Lane 1 contains nontransfected cells, while lane 2 contains cells transiently transfected with the human CD14 gene. Approximately 2 ug of lysate was loaded per lane. A prominent immunoreactive band is detected near 55 kDa in the CD14-transfected sample, consistent with the expected molecular weight of mature CD14. The corresponding signal is absent or substantially weaker in the nontransfected control. CD14 is a glycosylated protein, which can account for migration above its calculated polypeptide mass. This experiment provides useful transfection-based evidence supporting recognition of the Lipopolysaccharide Receptor.

CD14 Rabbit Polyclonal Antibody by IHC in Human Lung Carcinoma. Immunohistochemistry staining of FFPE human lung carcinoma tissue using rabbit polyclonal CD14 antibody. Brown immunoreactivity is visible throughout portions of the tissue, with prominent cytoplasmic staining and areas of increased intensity along cellular boundaries. The positively stained cells contain blue hematoxylin-counterstained nuclei, and the staining intensity varies across the field. This experiment demonstrates antibody reactivity in human lung carcinoma tissue. CD14, also known as the Lipopolysaccharide Receptor, is involved in innate immune recognition and may be expressed by different cell populations within the tumor microenvironment. The image alone does not establish the identity of the stained cells.

CD14 Rabbit Polyclonal Antibody by FACS in Human A549 Cells. Flow cytometry analysis of human A549 cells using rabbit polyclonal CD14 antibody, followed by FITC-conjugated goat anti-rabbit secondary antibody. The antibody-stained population (right histogram, green) exhibits a pronounced rightward fluorescence shift compared with the negative control (left histogram, blue). The distributions show clear separation, indicating increased antibody-associated fluorescence in the tested cells. This experiment supports the use of the antibody for detecting CD14-associated staining by flow cytometry. CD14 functions as a Lipopolysaccharide Receptor involved in bacterial endotoxin recognition and innate immune signaling.

CD14 Rabbit Polyclonal Antibody by WB in Human Liver and Lung Lysates. Western blot analysis of human liver lysate (lane 1) and human lung lysate (lane 2) using rabbit polyclonal CD14 antibody at a dilution of 1:2000. A prominent immunoreactive band is detected near 55-60 kDa in both samples, with the lung lysate producing a stronger signal. The observed migration is consistent with the expected molecular weight of mature CD14, which is approximately 40-55 kDa depending on glycosylation. CD14 contains multiple potential N-linked glycosylation sites, and differences in glycosylation may influence its electrophoretic mobility. These results demonstrate recognition of a protein consistent with the Lipopolysaccharide Receptor in two human tissue lysates.