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Home >> Antibodies >> CD14 Antibody (Macrophage Marker)

CD14 Antibody (Macrophage Marker) [clone CDLA14-2] (V3764)

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  Catalog No Formulation Size Price (USD)  
Image V3764-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3764-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3764SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2386). Expected molecular weight: 40-55 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CDLA14-2
Purity Protein G affinity chromatography
UniProt P08571
Localization Cell surface, Secreted, Cytoplasmic (Golgi)
Applications IHC (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This CD14 antibody is available for research use only.
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Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 25-148 was used as immunogen for this CD14 antibody.

Storage

Store the CD14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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