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Home >> Antibodies >> CD138 Antibody / Syndecan-1

CD138 Antibody / Syndecan-1 [clone 587CT7.3.6.5] (F53713)

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  Catalog No Formulation Size Price (USD)  
Image F53713-0.1ML In ascites with 0.09% sodium azide 0.1 ml 399
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CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
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CD138 antibody flow cytometric analysis of U266 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
CD138 antibody flow cytometric analysis of T47D cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
CD138 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary Ab was used for the analysis
Confocal immunofluorescent analysis of CD138 antibody with U266 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of CD138 antibody with T47D cells followed by secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of CD138 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
CD138 antibody western blot analysis in HepG2 lysate. Predicted molecular weight: 32-95 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM
Clone Name 587CT7.3.6.5
Purity Ascites
UniProt P18827
Applications Western blot : 1:500-1:8000
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This CD138 antibody is available for research use only.
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Description

CD138 is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The Syndecan-1/CD138 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins.

Application Notes

Titration of the CD138 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 210-238 from the human protein was used as the immunogen for this CD138 antibody.

Storage

Aliquot the CD138 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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