CD137 Antibody 4-1BB/3201 / 4-1BB Antibody [clone 4-1BB/3201] (V7567)

CD137 Antibody 4-1BB/3201 Detection in Human HEK293 Cells IF. Immunofluorescence staining of methanol-fixed human HEK293 cells using CD137 Antibody (clone 4-1BB/3201) shows strong green staining predominantly outlining the cell periphery, with red nuclear counterstaining providing cellular context. The distinct peripheral distribution is consistent with detection of the cell-surface receptor CD137. This result demonstrates detection of 4-1BB using the 4-1BB Antibody in human HEK293 cells.

CD137 Antibody 4-1BB/3201 Staining in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen using CD137 Antibody (clone 4-1BB/3201) shows heterogeneous brown staining distributed among cells throughout the splenic tissue. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The staining demonstrates detection of CD137 using this 4-1BB Antibody in human spleen.

CD137 Antibody 4-1BB/3201 Staining in Human Cerebellum IHC. Immunohistochemistry staining of FFPE human cerebellum using CD137 Antibody (clone 4-1BB/3201) shows prominent brown staining of scattered large neuronal cells, with additional lighter staining visible in the surrounding tissue. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The observed pattern demonstrates detection of CD137 using this 4-1BB Antibody in human cerebellum.

CD137 Antibody 4-1BB/3201 Staining in Human Renal Cell Carcinoma IHC. Immunohistochemistry staining of FFPE human renal cell carcinoma using CD137 Antibody (clone 4-1BB/3201) shows strong brown staining associated with tubular and gland-like epithelial structures, contrasting with comparatively weak staining in surrounding areas. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. The staining demonstrates detection using this 4-1BB Antibody in renal cell carcinoma tissue.

CD137 Antibody 4-1BB/3201 Staining in Human Renal Cell Carcinoma IHC. Immunohistochemistry staining of a second FFPE human renal cell carcinoma specimen using CD137 Antibody (clone 4-1BB/3201) shows intense heterogeneous brown staining within groups of tumor cells, with substantially weaker staining in adjacent areas. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling before testing. This second tumor specimen demonstrates an additional CD137 staining pattern obtained with the 4-1BB Antibody.

CD137 Antibody 4-1BB/3201 Detection in Human HEK293 Cells FACS. Flow cytometry analysis of methanol-fixed human HEK293 cells using CD137 Antibody (clone 4-1BB/3201) shows a clear rightward fluorescence shift of the antibody-stained population (blue) relative to the isotype control (red). The separation between the two distributions demonstrates detection of CD137 in HEK293 cells by FACS using this 4-1BB Antibody.

CD137 Antibody 4-1BB/3201 Protein Microarray Validated. Protein microarray analysis of CD137 Antibody (clone 4-1BB/3201) on a HuProt array containing more than 19,000 full-length human proteins identifies CD137 as the highest-ranked signal, with a Z-score of 95.34 and S-score of 74.05. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. The substantial separation of CD137 from the remaining proteins provides strong specificity evidence for this 4-1BB Antibody.

CD137 Antibody 4-1BB/3201 Detects CD137 in Human Kidney WB. Western blot analysis of human kidney lysate using CD137 Antibody (clone 4-1BB/3201) shows a strong, discrete band migrating at approximately 55-60 kDa. CD137 has a predicted monomeric molecular weight near 30 kDa, but extensive N-linked glycosylation can substantially increase its apparent molecular weight; dimeric CD137 species may also occur in this range. The observed band is therefore compatible with higher-molecular-weight CD137 detected using this 4-1BB Antibody.