CD137 Antibody / 4-1BB / TNFRSF9 [clone 4-1BB/3201] (V7567)

Immunofluorescence staining of MeOH-fixed human HEK293 cells with CD137 antibody (green, clone 4-1BB/3201) and Reddot nuclear stain (red).

IHC staining of FFPE human spleen with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of MeOH-fixed human HEK293 cells with CD137 antibody (clone 4-1BB/3201); Red=isotype control, Blue= CD137 mAb.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD137 antibody (clone 4-1BB/3201). These results demonstrate the foremost specificity of the 4-1BB/3201 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human kidney lysate with CD137 antibody. Expected molecular weight: ~30 kDa monomer and 55-60 kDa dimer. Higher molecular weights may be observed due to glycosylation.