CD127 Antibody / Interleukin 7 Receptor Antibody (RQ8854)

CD127 Antibody Detection in Human JK Cells FACS. Flow cytometry analysis of fixed human JK cells using CD127 Antibody shows a strong rightward fluorescence shift in the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat serum and tested with primary antibody at 1 ug/million cells. The well-separated populations demonstrate detection of cell-surface CD127 using this Interleukin 7 Receptor Antibody.

CD127 Antibody Detects IL7R in Human Lymphoid Cell Lines WB. Western blot analysis of human cell lysates using CD127 Antibody shows strong major bands at approximately 150 kDa. Lane 1: MOLT4 T lymphoblast cells; lane 2: K562 chronic myelogenous leukemia cells; lane 3: Jurkat T lymphocytes. The observed migration is consistent with the reported dimeric form of CD127, while glycosylated monomeric IL7R is expected at approximately 52-90 kDa. This Interleukin 7 Receptor Antibody produces a consistent, predominantly single-band pattern across all three lysates.

CD127 Antibody Staining in Human Colon Tissue IHC. Immunohistochemistry staining of FFPE human colon tissue using CD127 Antibody shows brown staining distributed among cells surrounding and between the colonic glands, with comparatively limited staining of the glandular epithelium. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. This Interleukin 7 Receptor Antibody demonstrates heterogeneous CD127 staining within the human colonic mucosa.

CD127 Antibody Detection in Human Colonic Mucosa IHC. Immunohistochemistry staining of FFPE human colon tissue using CD127 Antibody shows heterogeneous brown staining in cells within the lamina propria surrounding the colonic glands. The glandular epithelial structures are comparatively lightly stained. HIER was performed by boiling sections in pH 8 EDTA for 20 min followed by cooling before testing. This second field provides complementary visualization of CD127 using the Interleukin 7 Receptor Antibody in human colon tissue.

CD127 Antibody Staining in Human Colon Epithelium and Stroma IHC. Immunohistochemistry staining of FFPE human colon tissue using CD127 Antibody shows prominent brown staining along portions of the glandular epithelium together with numerous positive cells in the surrounding stromal compartment. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. This Interleukin 7 Receptor Antibody demonstrates a heterogeneous distribution of CD127 staining across epithelial and stromal regions.

CD127 Antibody Detection in Human Colon Glands and Stroma IHC. Immunohistochemistry staining of FFPE human colon tissue using CD127 Antibody shows distinct brown staining within portions of the glandular epithelium and numerous cells in the intervening stromal tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The staining provides an additional tissue field demonstrating detection of CD127 with this Interleukin 7 Receptor Antibody.