IL3RA Antibody / CD123 Antibody (RQ5983)

IL3RA Antibody in Human Raji B Lymphoma Cells FACS. Flow cytometry analysis of human Raji B lymphocytes using IL3RA Antibody at 1 ug/million cells demonstrates a clear rightward fluorescence shift of the antibody-stained population (blue) relative to the isotype control (green) and unstained cells (red). Cells were blocked with goat serum before staining. The well-separated positive population demonstrates cell-surface detection of IL3RA by this CD123 Antibody in Raji cells.

IL3RA Antibody in Human Rectal Cancer IHC. Immunohistochemistry of FFPE human rectal cancer tissue using IL3RA Antibody demonstrates prominent brown staining across tumor-cell populations, with membranous and cytoplasmic signal visible throughout the field. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The robust cellular staining demonstrates detection of IL3RA by this CD123 Antibody in human rectal cancer.

IL3RA Antibody Detects CD123 in Raji and K562 Cells WB. Western blot analysis of human Raji (lane 1) and K562 (lane 2) cell lysates using IL3RA Antibody demonstrates a strong, discrete band at approximately 55-60 kDa in both samples, with minimal additional background. This migration falls within the expected 43-70 kDa range reported for CD123 depending on glycosylation level. The consistent signal across both human hematopoietic cell lines demonstrates detection of IL3RA by this CD123 Antibody under Western blot conditions.