ITGAX Antibody / CD11c Polyclonal Antibody (F50002)

ITGAX Antibody Western Blot of MDA-MB-231 Cells. Western blot testing of human MDA-MB-231 cell lysate with ITGAX antibody. A major band is detected at approximately 120-130 kDa, near the expected 128-150 kDa molecular weight range reported for CD11c. A weaker lower-molecular-weight band is also visible near 55 kDa. The predominant high-molecular-weight signal supports detection of the ITGAX-encoded integrin alpha X protein by this CD11c polyclonal antibody.

ITGAX Antibody in SK-BR-3 Cells FACS. Flow cytometry analysis of human SK-BR-3 cells stained with ITGAX antibody and FITC-conjugated goat anti-rabbit secondary antibody. The CD11c-stained population shows a rightward fluorescence shift relative to the negative control, supporting detection of cell-associated integrin alpha X. This FACS result provides flow cytometric validation of the CD11c polyclonal antibody.

ITGAX Antibody in Human Hepatocarcinoma IF. Immunofluorescence analysis of paraffin-embedded human hepatocarcinoma tissue with ITGAX antibody followed by FITC-conjugated goat anti-rabbit IgG. Green fluorescence identifies CD11c immunoreactivity, while PI provides red nuclear counterstaining. The image shows heterogeneous green staining among cells within the tumor tissue, supporting detection of integrin alpha X by this CD11c polyclonal antibody.

ITGAX Antibody in Human Hepatocarcinoma IHC. Immunohistochemistry analysis of FFPE human hepatocarcinoma tissue with ITGAX antibody. Brown immunoreactivity is visible in numerous cells, with predominantly cytoplasmic and membranous-appearing staining and heterogeneous signal intensity across the field. The staining demonstrates tissue-based detection of CD11c and complements the other application data available for this ITGAX antibody.