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Home >> Antibodies >> CD11c Antibody / ITGAX

CD11c Antibody / ITGAX (F50002)

  Catalog No Formulation Size Price (USD)  
Image F50002-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50002-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of CD11c antibody and MDA-MB231 lysate. Predicted molecular weight: 128~150 kDa.
Flow cytometric analysis of SK-Br-3 cells using CD11c antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Immunofluorescence analysis of CD11c antibody with paraffin-embedded human hepatocarcinoma. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
IHC analysis of FFPE human hepatocarcinoma stained with CD11c antibody
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P20702
Localization Cell surface, cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This CD11c antibody is available for research use only.
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Description

ITGAX is the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles.

Application Notes

Titration of the CD11c antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1119-1145 from the human protein was used as the immunogen for this CD11c antibody.

Storage

Aliquot the CD11c antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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