CD117 Antibody C117/6347 / c-Kit Monoclonal Antibody [clone C117/6347] (V5050)

CD117 Antibody C117/6347 Protein Microarray Validation. Protein microarray analysis of CD117 Antibody clone C117/6347 using a HuProt array containing more than 19,000 full-length human proteins. KIT produces the dominant signal, clearly separated from the next-highest array proteins. The Z-score represents target signal strength in standard deviations above the mean signal across the array, while the S-score measures the difference between the target Z-score and the next-highest signal. The strong separation of KIT from other proteins demonstrates excellent target specificity and supports this c-Kit Monoclonal Antibody as Protein Microarray Validated.

CD117 Antibody C117/6347 in Human GIST IHC. Immunohistochemistry staining of FFPE human gastrointestinal stromal tumor (GIST) tissue with CD117 Antibody clone C117/6347. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. Strong, widespread brown immunoreactivity is visible throughout the tumor-cell population, with comparatively pale nuclei. The robust staining in this highly relevant CD117-positive tumor context demonstrates strong tissue performance of the c-Kit Monoclonal Antibody.

CD117 Antibody C117/6347 Purity by SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free CD117 Antibody clone C117/6347 under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, prominent bands are visible near the expected approximately 50 kDa heavy-chain and 25 kDa light-chain positions. Under non-reducing conditions, the intact antibody migrates predominantly as a high-molecular-weight species near approximately 150 kDa. The expected change in migration between reducing and non-reducing conditions confirms the integrity and purity of the c-Kit Monoclonal Antibody preparation.